Isolation of Human Eosinophils

Neurotoxicity of human eosinophils.

Eosinophils contain a substance that is neurotoxic when injected intracerebrally or intrathecally into laboratory animals-an effect known as the "Gordon phenomenon." We found neurotoxic activity in eosinophils from three patients with eosinophilic syndromes by injecting cell preparations into rabbits and guinea pigs. These animals developed a syndrome of muscular rigidity and ataxia, progressin...

Subcellular fractionation of human eosinophils: isolation of functional specific granules on isoosmotic density gradients.

Subcellular fractionation has been an important tool in investigating human eosinophil structure and function, including localizing of cytokine/chemokines within granules, investigating granule protein translocation and intracellular transport during eosinophil secretion, and studying secretory mechanisms of granules. The resolution of organelles obtained by subcellular fractionation was improv...

Phagocytosis by human eosinophils.

E OSINOPHILS were first recognized as a distinctive type of granulocyte over 100 years ago,’ and were observed to be capable of phagocytosis by Mesnil in 1895.2 Despite a voluminous literature on the morphology and functions of these cells, their precise role in normal man and in various diseases remains unclear. In general, eosinophils from higher mammals resemble other granulocytic cells in t...

Activated human eosinophils.

Surface markers of human eosinophils.

Peripheral blood eosinophils from patients with eosinophilia and from healthy subjects were studied for surface immunoglobulins, receptors for the Fc region of IgG, complement receptors, and spontaneous rosette formation with sheep and mouse erythrocytes. Eosinophils were found to have receptors for complement and for aggregated IgG, and to have the same two types of complement receptors as do ...